FOXG1 Recombinant Rabbit mAb (S-5244)
Forkhead box protein G1,Brain factor 1 (BF-1,BF1),Brain factor 2 (BF-2,BF2,hBF-2),Forkhead box protein G1A,Forkhead box protein G1B,Forkhead box protein G1C,FKH2,FKHL1,FKHL2,FKHL3,FKHL4,FOXG1A,FOXG1B,FOXG1C
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宿主来源
Rabbit抗原名称
FOXG1分子别名
Forkhead box protein G1; Brain factor 1 (BF-1; BF1); Brain factor 2 (BF-2; BF2; hBF-2); Forkhead box protein G1A; Forkhead box protein G1B; Forkhead box protein G1C; FKH2; FKHL1; FKHL2; FKHL3; FKHL4; FOXG1A; FOXG1B; FOXG1C细胞定位
NucleusAccession
P55316克隆号
S-5244抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
NCI-H69, HEK-293, U-87 MG, mouse brain, C6, rat brain纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:2000 Hu, Ms, Rt
FOXG1 (Forkhead box G1) is a winged-helix transcription factor essential for vertebrate forebrain development, acting as a pioneer factor that opens chromatin and orchestrates the earliest steps of telencephalon specification by repressing dorsalizing genes, promoting FGF8 expression, and directing the temporal progression of cortical progenitor proliferation, neuronal layer fate transitions, oligodendrocyte maturation and postnatal myelination through dosage-sensitive interactions with morphogen signals, histone-modifying complexes and post-translational phosphorylation that control its nuclear–cytoplasmic shuttling, so that even modest alterations in FOXG1 levels produce a spectrum of neurodevelopmental disorders ranging from microcephaly and corpus callosum agenesis to severe encephalopathy with autism-like behaviors.
免疫印迹
WB result of FOXG1 Recombinant Rabbit mAb
Primary antibody: FOXG1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NCI-H69 whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Lane 3: U-87 MG whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 58 kDaWB result of FOXG1 Recombinant Rabbit mAb
Primary antibody: FOXG1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 58 kDa
This blot was developed with high sensitivity substrateWB result of FOXG1 Recombinant Rabbit mAb
Primary antibody: FOXG1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 58 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human glioma. Anti-FOXG1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-FOXG1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-FOXG1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat E14 embryo. Anti-FOXG1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of FOXG1 in tumor tissues.
Expression of FOXG1 in human tissues.
Expression of FOXG1 in mouse & rat tissues.
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