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SDHA Recombinant Rabbit mAb (S-1458-43)

Succinate dehydrogenase [ubiquinone] flavoprotein subunit,mitochondrial,Flavoprotein subunit of complex II (Fp),Malate dehydrogenase [quinone] flavoprotein subunit,SDH2,SDHF

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6004

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

SDHA
分子别名

Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial; Flavoprotein subunit of complex II (Fp); Malate dehydrogenase [quinone] flavoprotein subunit; SDH2; SDHF
免疫原

Synthetic Peptide
细胞定位

Mitochondrion
Accession

P31040
克隆号

S-1458-43
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

HepG2, Jurkat, HT-1080, HeLa, MCF7, mouse kidney, rat kidney
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IHC-P 1:250 Hu, Ms, Rt

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IHC-P	1:250	Hu, Ms, Rt

背景介绍

SDHA protein, encoded by the SDHA gene, is a crucial component of the succinate dehydrogenase complex, which is involved in the mitochondrial respiratory chain and the citric acid cycle. This protein acts as a catalytic subunit, facilitating the conversion of succinate to fumarate and transferring electrons to the oxidative phosphorylation pathway. It contains a covalently attached flavin adenine dinucleotide (FAD) cofactor, which is essential for its enzymatic activity. Mutations in the SDHA gene can lead to various disorders, including mitochondrial complex II deficiency, Leigh syndrome, and paraganglioma-pheochromocytoma syndrome. These mutations may result in impaired mitochondrial function, reduced ATP production, and the accumulation of succinate, which can disrupt cellular metabolism and contribute to disease development.

免疫印迹
- WB result of SDHA Recombinant Rabbit mAb
  Primary antibody: SDHA Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HepG2 whole cell lysate 20 µg
  Lane 2: Jurkat whole cell lysate 20 µg
  Lane 3: HT-1080 whole cell lysate 20 µg
  Lane 4: HeLa whole cell lysate 20 µg
  Lane 5: MCF7 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 73 kDa
  Observed MW: 68 kDa
- WB result of SDHA Recombinant Rabbit mAb
  Primary antibody: SDHA Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: mouse kidney lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 73 kDa
  Observed MW: 68 kDa
- WB result of SDHA Recombinant Rabbit mAb
  Primary antibody: SDHA Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: rat kidney lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 73 kDa
  Observed MW: 68 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human kidney. Anti-SDHA antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human liver. Anti-SDHA antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human stomach. Anti-SDHA antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon cancer. Anti-SDHA antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human prostatic cancer. Anti-SDHA antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-SDHA antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat stomach. Anti-SDHA antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
- Expression of SDHA in tumor tissue.
- Expression of SDHA in human tissue.
- Expression of SDHA in mouse & rat tissue.