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Rabbit抗原名称
ALPI分子别名
Intestinal-type alkaline phosphatase; IAP; Intestinal alkaline phosphataseAlternative nameAlkaline phosphatase 3; Akp-3; Akp3免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
P24822克隆号
S-3768-104抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Ms, Rt阳性样本
mouse small intestine纯化方式
Protein A浓度
0.25 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms, Rt IHC-P 1:2500 Ms, Rt
ALPI (intestinal alkaline phosphatase) protein is a homodimeric glycoprotein primarily expressed on the brush border of the small intestinal mucosa, encoded by a gene located on human chromosome 2q37.1, and belongs to an important member of the tissue-nonspecific alkaline phosphatase family. As a core component of the intestinal mucosal barrier, ALPI not only participates in the digestion and absorption of nutrients by hydrolyzing phosphate esters but, more critically, catalyzes the dephosphorylation of lipopolysaccharide (LPS) and endogenous nucleotides, thereby exerting endotoxin detoxification and anti-inflammatory effects, effectively inhibiting bacterial translocation and intestinal inflammation. At the molecular level, ALPI maintains the intestinal epithelial mechanical barrier by enhancing the expression of tight junction proteins (such as ZO-1 and Occludin) and regulates the TLR4/NF-κB signaling pathway to sustain immune homeostasis. Recent studies have also revealed that ALPI expression is regulated by various physiological and pathological conditions, such as complement C3 deficiency, and its upregulation can restore the expression of mucin-related genes and improve the constipation phenotype. Furthermore, mutations or aberrant expression of the ALPI gene are not only closely associated with inflammatory bowel disease and necrotizing enterocolitis but have also been found to affect glycolipid metabolism, suggesting that this protein plays a critical role in maintaining intestinal homeostasis and preventing metabolic diseases.
免疫印迹
WB result of ALPI Recombinant Rabbit mAb
Primary antibody: ALPI Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse kidney lysate 20 µg
Lane 2: mouse small intestine lysate 20 µg
Negative control: mouse kidney lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60 kDa
Observed MW: 80 kDa
免疫组化
IHC shows positive staining in paraffin-embedded mouse small intestine. Anti-ALPI antibody was used at 1/2500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded mouse colon. Anti-ALPI antibody was used at 1/2500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat small intestine. Anti-ALPI antibody was used at 1/2500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded rat colon. Anti-ALPI antibody was used at 1/2500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of ALPI in mouse & rat tissues.
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