ACSS2 Recombinant Rabbit mAb (S-4117-9)
Acetyl-coenzyme A synthetase,cytoplasmic,Acetate--CoA ligase,Acetyl-CoA synthetase (ACS,AceCS),Acetyl-CoA synthetase 1 (AceCS1),Acyl-CoA synthetase short-chain family member 2,Acyl-activating enzyme,Propionate--CoA ligase,ACAS2
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宿主来源
Rabbit抗原名称
ACSS2分子别名
Acetyl-coenzyme A synthetase, cytoplasmic; Acetate--CoA ligase; Acetyl-CoA synthetase (ACS; AceCS); Acetyl-CoA synthetase 1 (AceCS1); Acyl-CoA synthetase short-chain family member 2; Acyl-activating enzyme; Propionate--CoA ligase; ACAS2免疫原
Synthetic Peptide细胞定位
Cytoplasm, NucleusAccession
Q9NR19克隆号
S-4117-9抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HepG2, Caco-2, U-87 MG, Neuro-2a, mouse liver, mouse brain, rat brain, rat liver, COS-7纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:500-1:1000 Hu, Ms, Rt IHC-P 1:200 Hu, Ms, Rt
ACSS2 (Acyl-CoA Synthetase Short-Chain Family Member 2) is a metabolic enzyme primarily localized in the cytoplasm, whose core function is to catalyze the conversion of acetate into acetyl-CoA, thereby connecting metabolism with lipid synthesis, energy production, and gene expression regulation. In its classical role, ACSS2 provides substrates for histone acetylation by generating acetyl-CoA, thus epigenetically regulating gene transcription; however, recent studies have revealed that its functions extend far beyond this. It can directly acetylate metabolic enzymes such as PAICS to influence purine synthesis and DNA repair, thereby regulating the senescence-associated secretory phenotype (SASP). Moreover, upon responding to oncogenic signals, ACSS2 can be phosphorylated, translocate to the nucleus, and utilize lactate as a substrate to synthesize lactyl-CoA, collaborating with KAT2A to catalyze histone lactylation, thereby promoting tumor immune evasion. Additionally, in alcoholic liver disease, the downregulation of ACSS2 epigenetically inhibits hepcidin expression, inducing ferroptosis and liver injury. These findings reveal ACSS2 as a critical metabolic-epigenetic hub that, through its substrate diversity and functional plasticity, plays a complex and important role in aging, inflammation, and tumor development and progression.
免疫印迹
WB result of ACSS2 Recombinant Rabbit mAb
Primary antibody: ACSS2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Raji whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: Caco-2 whole cell lysate 20 µg
Lane 4: U-87 MG whole cell lysate 20 µg
Low expression control: Raji whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 78 kDa
Observed MW: 78 kDaWB result of ACSS2 Recombinant Rabbit mAb
Primary antibody: ACSS2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Neuro-2a whole cell lysate 20 µg
Lane 2: mouse liver lysate 20 µg
Lane 3: mouse brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 78 kDa
Observed MW: 78 kDaWB result of ACSS2 Recombinant Rabbit mAb
Primary antibody: ACSS2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat liver lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 78 kDa
Observed MW: 78 kDaWB result of ACSS2 Recombinant Rabbit mAb
Primary antibody: ACSS2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 78 kDa
Observed MW: 78 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-ACSS2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-ACSS2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-ACSS2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-ACSS2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of ACSS2 in tumor tissues.
Expression of ACSS2 in human tissues.
Expression of ACSS2 in mouse & rat tissues.
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