分享产品介绍 评论(0)
宿主来源
Rabbit抗原名称
YY1分子别名
Transcriptional repressor protein YY1; Delta transcription factor; INO80 complex subunit S; NF-E1; Yin and yang 1 (YY-1); INO80S免疫原
Synthetic Peptide细胞定位
NucleusAccession
P25490克隆号
S-3652-44抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
HeLa, Daudi, Jurkat, MDA-MB-231, T-47D, MCF7纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:2000 Hu
YY1 is a ubiquitously expressed multifunctional zinc finger transcription factor belonging to the GLI-Krüppel family, named for its dual "yin-yang" regulatory property of both activating and repressing target gene transcription. The key structural feature of this protein is its C-terminal region containing four tandem C2H2-type zinc finger domains responsible for sequence-specific recognition and binding to the core DNA motif (5'-CCGCCATN-3'), while the N-terminal histidine/proline-rich region and acidic domain mediate interactions with various transcriptional cofactors (such as p300 and HDACs) and epigenetic regulatory proteins. YY1 plays a central regulatory network role in cell biology, participating in numerous physiological processes including embryonic development, cell proliferation, differentiation, apoptosis, DNA repair, and viral replication. Its multifunctionality is reflected in the following: on one hand, it represses target gene transcription by directly occupying promoter regions or recruiting histone deacetylases; on the other hand, it activates gene expression by recruiting histone acetyltransferases or cooperating with other transcription factors (such as Myc and p53). YY1 also plays an important architectural role, mediating the formation of DNA loops that bring distant regulatory elements (enhancers and promoters) into close proximity, thereby coordinating the expression of complex gene loci. Clinical studies have shown that YY1 is overexpressed in most tumors (such as lymphoma, breast cancer, prostate cancer, and pancreatic cancer), promoting tumor proliferation, invasion, metastasis, and chemoresistance by regulating epithelial-mesenchymal transition, glycolytic metabolic reprogramming, and anti-apoptotic genes. Conversely, in certain tumors, YY1 can also function as a tumor suppressor. Additionally, YY1 gene mutations are associated with a rare X-linked recessive disorder—Gabriele-de Vries syndrome, characterized by developmental delay, intellectual disability, and congenital abnormalities. YY1 is also involved in regulating pluripotent stem cell self-renewal and somatic cell reprogramming, serving as a key factor in maintaining embryonic stem cell identity.
免疫印迹
WB result of YY1 Recombinant Rabbit mAb
Primary antibody: YY1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Daudi whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: MDA-MB-231 whole cell lysate 20 µg
Lane 5: T-47D whole cell lysate 20 µg
Lane 6: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 45 kDa
Observed MW: 65 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-YY1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-YY1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-YY1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-YY1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-YY1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach cancer. Anti-YY1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-YY1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of YY1 in tumor tissues.
Expression of YY1 in human tissues.
评论(0)