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Runx2 Recombinant Rabbit mAb (S-1767-173)

Runt-related transcription factor 2,Acute myeloid leukemia 3 protein,Core-binding factor subunit alpha-1 (CBF-alpha-1),Oncogene AML-3,Osteoblast-specific transcription factor 2 (OSF-2),Polyomavirus enhancer-binding protein 2 alpha A subunit (PEA2-alpha A,PEBP2-alpha A),AML3,CBFA1,OSF2,PEBP2A

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B6003
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产品介绍

产品介绍 FAQs 引用文献(2) 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Runx2

  • 分子别名

    Runt-related transcription factor 2; Acute myeloid leukemia 3 protein; Core-binding factor subunit alpha-1 (CBF-alpha-1); Oncogene AML-3; Osteoblast-specific transcription factor 2 (OSF-2); Polyomavirus enhancer-binding protein 2 alpha A subunit (PEA2-alpha A; PEBP2-alpha A); AML3; CBFA1; OSF2; PEBP2A

  • 免疫原

    Recombinant Protein

  • 细胞定位

    Nucleus, Cytoplasm

  • Accession

    Q13950

  • 克隆号

    S-1767-173

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    Saos-2, PC-3, MDA-MB-231, C2C12

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    ChIP

    ICFCM

    IHC-P ?

    ICC ?

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu, Ms
    IHC-P 1:500 Hu, Ms, Rt
    ICC 1:500 Hu, Ms
    ICFCM 1:50 Hu
    ChIP 1:20-1:50 Hu
背景介绍

  • Runx2, also known as Runt-related transcription factor 2, is a crucial transcription factor for bone development and plays a key role in the regulation of osteoblast differentiation and skeletal morphogenesis. It is expressed in prehypertrophic and hypertrophic chondrocytes, and its mutation can lead to skeletal malformation syndromes such as cleidocranial dysplasia. Runx2 induces chondrocyte maturation and proliferation through the regulation of various signaling pathways, including Indian hedgehog (Ihh) and vascular endothelial growth factor A (Vegfa). In addition to its role in normal bone development, Runx2 is also involved in pathological conditions such as breast cancer metastasis and lung fibrosis.

  • 免疫印迹

    • WB result of Runx2 Recombinant Rabbit mAb
      Primary antibody: Runx2 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: 293T whole cell lysate 20 µg
      Lane 2: LNCaP whole cell lysate 20 µg
      Lane 3: Saos-2 whole cell lysate 20 µg
      Lane 4: PC-3 whole cell lysate 20 µg
      Lane 5: MDA-MB-231 whole cell lysate 20 µg
      Lane 6: HeLa whole cell lysate 20 µg
      Negative control: 293T whole cell lysate
      Low expression control: LNCaP whole cell lysate
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 57 kDa
      Observed MW: 50-70 kDa

    • WB result of Runx2 Recombinant Rabbit mAb
      Primary antibody: Runx2 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: C2C12 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 57 kDa
      Observed MW: 50-70 kDa

  • 流式分析

    • Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized LNCAP (Human prostate carcinoma epithelial cell, left) / PC-3 (Human prostate adenocarcinoma epithelial cell, right) labelling Runx2 antibody at 1/50 dilution (1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
      Negative control: LNCAP

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human tonsil. Anti-Runx2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human prostatic hyperplasia. Anti-Runx2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-Runx2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-Runx2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human osteosarcoma. Anti-Runx2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse spleen. Anti-Runx2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat spleen. Anti-Runx2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in PC-3 cells (top panel) and negative staining in LNCaP cells (below panel). Anti-Runx2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • ChIP

    • Chromatin immunoprecipitation (ChIP) was performed on PC-3 cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication.
      Parallel reactions used Runx2Recombinant Rabbit mAb (S-1767-173) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
      Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
      qPCR showed the enrichment of RUNX1, RUNX2 and SAT-α in Runx2 Recombinant
      Rabbit mAb (S-1767-173)-immunoprecipitated sample.

  • 组织表达图谱

    • Expression of Runx2 in tumor tissue.

    • Expression of Runx2 in human tissue.

    • Expression of Runx2 in mouse & rat tissue.

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

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