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Rabbit抗原名称
RIBEYE/CTBP2分子别名
C-terminal-binding protein 2免疫原
Synthetic Peptide细胞定位
Nucleus, SynapseAccession
P56545抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, SK-BR-3, SH-SY5Y, MCF7, mouse brain, mouse stomach, rat brain, rat stomach预测反应种属
(反应种属缩写表)Bv纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:1000 Hu, Ms, Rt ICC 1:1000 Hu
The RIBEYE protein is a core structural component of the synaptic ribbon in retinal bipolar cells and photoreceptor cells. It is one of two isoforms (the shorter B-domain-deficient variant) encoded by the CTBP2 gene through an alternative promoter. Functioning as a scaffold protein of the synaptic ribbon, RIBEYE interacts with other synaptic components (such as RIM and Piccolo) via its unique N-terminal A-domain, while its C-terminal D-domain—homologous to CTBP1/2—mediates dimerization and may participate in metabolic regulation. This protein plays a crucial role in synaptic vesicle tethering, organizing release sites, and coordinating calcium signaling. Mutations in RIBEYE are associated with retinal disorders and hearing impairments. Research on RIBEYE provides insights into the mechanisms of specialized synaptic transmission and the pathological basis of neuro-sensory diseases.
免疫印迹
WB result of RIBEYE/CTBP2 Rabbit pAb
Primary antibody: RIBEYE/CTBP2 Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: SK-BR-3 whole cell lysate 20 µg
Lane 3: SH-SY5Y whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 49 kDa
Observed MW: 49 kDaWB result of RIBEYE/CTBP2 Rabbit pAb
Primary antibody: RIBEYE/CTBP2 Rabbit pAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Lane 2: mouse stomach lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 49 kDa
Observed MW: 49 kDaWB result of RIBEYE/CTBP2 Rabbit pAb
Primary antibody: RIBEYE/CTBP2 Rabbit pAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Lane 2: rat stomach lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 49 kDa
Observed MW: 49 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-RIBEYE/CTBP2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-RIBEYE/CTBP2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-RIBEYE/CTBP2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-RIBEYE/CTBP2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti-RIBEYE/CTBP2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in SK-BR-3 cells. Anti- RIBEYE/CTBP2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of RIBEYE/CTBP2 in tumor tissue.
Expression of RIBEYE/CTBP2 in human tissue.
Expression of RIBEYE/CTBP2 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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