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Rabbit抗原名称
DLX3分子别名
Homeobox protein DLX-3免疫原
Synthetic Peptide细胞定位
Cytoplasm, NucleusAccession
O60479克隆号
S-4140-2抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
JAR纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms IHC-P 1:2000 Hu, Ms, Rt ICC 1:500 Hu
DLX3 (Distal-less 3 homeobox protein) is a homeodomain-containing transcription factor belonging to the DLX family, playing critical regulatory roles in embryonic development, skeletal formation, and tissue differentiation. The core structural feature of this protein is its highly conserved homeodomain, which specifically recognizes and binds to DNA sequences in the promoter regions of downstream target genes, thereby activating or repressing gene transcription. The expression pattern of DLX3 exhibits significant tissue specificity, being predominantly enriched in the placenta, tooth follicles, and bone tissue, where it finely regulates the differentiation and maturation of osteoblasts, odontoblasts, and trophoblast cells through synergistic interactions with signaling pathways such as BMP and WNT. Clinical and genetic studies have shown that gain-of-function or loss-of-function mutations in the DLX3 gene can lead to a spectrum of autosomal dominant disorders, including tricho-dento-osseous syndrome and amelogenesis imperfecta with taurodontism, which are primarily characterized by enamel mineralization defects, skeletal abnormalities, and sparse curly hair. Furthermore, DLX3 also plays important roles in osteoclastogenesis and epidermal barrier formation, and its dysregulation may be associated with the development of osteoporosis and certain skin diseases, underscoring the research value of this transcription factor in developmental biology and regenerative medicine.
免疫印迹
WB result of DLX3 Recombinant Rabbit mAb
Primary antibody: DLX3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: JAR whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 32 kDa
Observed MW: 38 kDaWB result of DLX3 Recombinant Rabbit mAb
Primary antibody: DLX3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse skin lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 32 kDa
Observed MW: 40 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti-DLX3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse skin. Anti-DLX3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat skin. Anti-DLX3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in JAR cells. Anti- DLX3 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of DLX3 in tumor tissues.
Expression of DLX3 in human tissues.
Expression of DLX3 in mouse & rat tissues.
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