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宿主来源
Rabbit抗原名称
CD1c分子别名
T-cell surface glycoprotein CD1c; CD1C细胞定位
Cell membrane, Endosome, LysosomeAccession
P29017克隆号
S-5242抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
Jurkat, Molt-4纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:500 Hu
CD1c is a lipid-presenting, MHC class I-like glycoprotein expressed on dendritic cells and B cells that binds both self and foreign lipids/glycolipids within its deep hydrophobic groove and shuttles them through endosomal compartments to display them to αβ and γδ T cells, thereby bridging innate and adaptive immunity; uniquely, its open F′ pocket can accommodate both lipid tails and peptide moieties of lipopeptides, enabling recognition of mycobacterial antigens such as mannosyl-β1-phosphomycoketide and driving potent IFN-γ responses essential for controlling intracellular pathogens like Mycobacterium tuberculosis.
免疫印迹
WB result of CD1c Rabbit Recombinant Rabbit mAb
Primary antibody: CD1c Rabbit Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: THP-1 whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: Molt-4 whole cell lysate 20 µg
Negative control: THP-1 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 37 kDa
Observed MW: 39 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human spleen. Anti-CD1c antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thymus. Anti-CD1c antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD1c antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of CD1c in tumor tissues.
Expression of CD1c in human tissues.
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