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宿主来源
Rabbit抗原名称
CCDC47分子别名
PAT complex subunit CCDC47; Calumin; Coiled-coil domain-containing protein 47免疫原
Synthetic Peptide细胞定位
Endoplasmic reticulumAccession
Q96A33克隆号
S-4158-17抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
HeLa, Jurkat, HEK-293纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:500 Hu
CCDC47 (Coiled-Coil Domain Containing Protein 47) is a multifunctional transmembrane protein primarily localized to the endoplasmic reticulum, playing a critical role in cellular calcium homeostasis regulation, protein processing, and the unfolded protein response. This protein is characterized by an N-terminal signal peptide, a central region containing multiple predicted coiled-coil domains that mediate protein-protein interactions, and a C-terminal transmembrane domain that anchors it to the endoplasmic reticulum membrane. As an important member of endoplasmic reticulum calcium-binding proteins, CCDC47 binds calcium ions and participates in regulating calcium store homeostasis within the ER lumen, thereby influencing calcium-dependent signaling and apoptotic processes. Additionally, this protein is involved in ER-associated protein degradation pathways and the formation of ER-mitochondria contact sites, helping to maintain functional coupling between organelles. Genetic studies have revealed that mutations in the CCDC47 gene are directly associated with a rare progressive neurological disorder—early-onset spastic paraplegia with optic atrophy and cognitive impairment (SPOAN syndrome)—highlighting the irreplaceable protective role of this protein in neurodevelopment and myelination processes.
免疫印迹
WB result of CCDC47 Recombinant Rabbit mAb
Primary antibody: CCDC47 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: HEK-293 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 65 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-CCDC47 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cerebellum. Anti-CCDC47 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-CCDC47 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-CCDC47 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-CCDC47 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostatic cancer. Anti-CCDC47 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-CCDC47 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of CCDC47 in tumor tissues.
Expression of CCDC47 in human tissues.
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