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宿主来源
Rabbit抗原名称
Aass分子别名
Alpha-aminoadipic semialdehyde synthase, mitochondrial; AASS免疫原
Synthetic Peptide细胞定位
MitochondrionAccession
Q9UDR5克隆号
S-4170-22抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
293T, HepG2, HUVEC, HeLa纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:500-1:2000 Hu, Ms, Rt
Aass (alpha-aminoadipic semialdehyde synthase, also known as lysine-ketoglutarate reductase/saccharopine dehydrogenase) is a bifunctional enzyme localized to the mitochondria and plays a critical role in the lysine degradation pathway. This enzyme consists of two independent domains: its N-terminal domain possesses lysine-ketoglutarate reductase activity, catalyzing the conversion of lysine and α-ketoglutarate into saccharopine; while its C-terminal domain exhibits saccharopine dehydrogenase activity, further converting saccharopine into α-aminoadipic semialdehyde, thereby completing the first two steps of lysine catabolism. Dysfunction of this gene directly leads to lysine metabolic disorders, resulting in hyperlysinemia. Notably, when the saccharopine dehydrogenase domain is specifically impaired, it causes abnormal accumulation of the metabolic intermediate saccharopine in the body, which subsequently disrupts mitochondrial homeostasis and affects organismal development.
免疫印迹
WB result of Aass Recombinant Rabbit mAb
Primary antibody: Aass Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: HUVEC whole cell lysate 20 µg
Lane 4: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 102 kDa
Observed MW: 110 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-Aass antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-Aass antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-Aass antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-Aass antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-Aass antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat liver. Anti-Aass antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Aass in tumor tissues.
Expression of Aass in human tissues.
Expression of Aass in mouse & rat tissues.
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