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Rabbit抗原名称
AARS1分子别名
Alanine--tRNA ligase, cytoplasmic; Alanyl-tRNA synthetase (AlaRS); Protein lactyltransferase AARS1; Renal carcinoma antigen NY-REN-42; AARS细胞定位
Cytoplasm, NucleusAccession
P49588抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
MCF7, A549, HeLa, HepG2, Raji, NIH/3T3, C6纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt ICC 1:500 Hu
AARS1 (Alanyl-tRNA synthetase 1) is a highly evolutionarily conserved class II aminoacyl-tRNA synthetase, whose core function is to catalyze the ligation of alanine to its corresponding tRNA(Ala), thereby providing critical quality control for protein synthesis. This enzyme accomplishes its task through a two-step process: first, it activates alanine using ATP to form alanyl-AMP; second, it transfers the activated alanine to tRNA (Ala). To ensure absolute translational fidelity, AARS1 is also equipped with a built-in editing or proofreading domain, which hydrolyzes and corrects mischarged amino acids, thus preventing the synthesis of toxic proteins caused by errors in genetic information translation. Although AARS1 is widely expressed throughout the body and primarily localizes to the cytoplasm, its dysfunction exhibits remarkable tissue specificity—particularly within the nervous system, where mutations in this gene have been definitively linked to several severe neurodegenerative diseases, such as Charcot-Marie-Tooth disease (CMT2N) and developmental epileptic encephalopathy (DEE29).
免疫印迹
WB result of AARS1 Rabbit pAb
Primary antibody: AARS1 Rabbit pAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: A549 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 107 kDa
Observed MW: 110 kDaWB result of AARS1 Rabbit pAb
Primary antibody: AARS1 Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 107 kDa
Observed MW: 110 kDaWB result of AARS1 Rabbit pAb
Primary antibody: AARS1 Rabbit pAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 107 kDa
Observed MW: 110 kDa
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti-AARS1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in HepG2 cells. Anti-AARS1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in Raji cells. Anti-AARS1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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