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Phospho-NRF2 (Ser40) Recombinant Rabbit mAb (S-1720-97)

Nuclear factor erythroid 2-related factor 2,NF-E2-related factor 2,NFE2-related factor 2,Nrf-2,Nuclear factor,erythroid derived 2,like 2,NFE2L2

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6001

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

Phospho-NRF2 (Ser40)
分子别名

Nuclear factor erythroid 2-related factor 2; NF-E2-related factor 2; NFE2-related factor 2; Nrf-2; Nuclear factor; erythroid derived 2, like 2; NFE2L2
免疫原

Synthetic Peptide
细胞定位

Nucleus
Accession

Q16236
克隆号

S-1720-97
抗体类型

Recombinant mAb
抗体同种型

IgG
翻译后修饰类型

磷酸化
反应种属 ?

Hu
阳性样本

HeLa
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB
稀释度

应用稀释度推荐种属

Dot Blot 1:1000
WB 1:250 Hu
IHC-P 1:500 Hu

应用	稀释度	推荐种属
Dot Blot	1:1000
WB	1:250	Hu
IHC-P	1:500	Hu

背景介绍

Phospho-NRF2 (Ser40) is a crucial form of the NRF2 protein, which is a basic leucine zipper transcription factor involved in regulating the antioxidant response. When phosphorylated at Ser40 by protein kinase C (PKC), NRF2 dissociates from its repressor KEAP1, leading to its nuclear translocation and activation. This activation results in the upregulation of antioxidant genes containing antioxidant response elements (AREs), helping cells combat oxidative stress. The phosphorylation of NRF2 at Ser40 is essential for its stability and function, and it plays a significant role in various cellular processes, including protection against oxidative damage and regulation of metabolic pathways.

免疫印迹
- WB result of Phospho-NRF2 (Ser40) Recombinant Rabbit mAb
  Primary antibody: Phospho-NRF2 (Ser40) Recombinant Rabbit mAb at 1/250 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: phosphatase treated HeLa whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 68 kDa
  Observed MW: 110 kDa
斑点杂交
- Dot blot result of Phospho-NRF2 (Ser40) Recombinant Rabbit mAb
  Lane 1: NRF2 (Ser40) phospho peptide 1
  Lane 2: NRF2 (Ser40) phospho peptide 2
  Lane 3: NRF2 (Ser40) unmodified peptide
  Primary antibody: Phospho-NRF2 (Ser40) Recombinant Rabbit mAb at 1/1000 dilution
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
免疫组化
- IHC shows positive staining in paraffin-embedded human colon (Left) and negative staining in human colon treated with phosphatase (Right). Anti- Phospho-NRF2 (Ser40) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung adenocarcinoma (Left) and negative staining in human lung adenocarcinoma treated with phosphatase (Right). Anti- Phospho-NRF2 (Ser40) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.