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宿主来源
Rabbit抗原名称
WSB1分子别名
WD repeat and SOCS box-containing protein 1; SWIP1免疫原
Synthetic Peptide细胞定位
CytoplasmAccession
Q9Y6I7克隆号
S-3505-23抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:250 Hu
WSB1 (WD repeat and SOCS box-containing protein 1) is an adaptor protein encoded by a gene on chromosome 17 in humans, functioning primarily as a substrate recognition subunit of the Cullin-RING E3 ubiquitin ligase complex (CRL). Its protein structure features two main functional modules: multiple WD40 repeats covering most of the sequence and a C-terminal SOCS box. The WD40 repeats are responsible for specifically recognizing and binding substrate proteins, while the SOCS box mediates interactions with core complex subunits such as Elongin B/C and Cullin-5, collectively catalyzing substrate ubiquitination and promoting their proteasomal degradation. Known degradation substrates of WSB1 include type 2 iodothyronine deiodinase (DIO2) and methylated RELA (p65), positioning it as a key player in regulating thyroid hormone metabolism and in terminating inflammatory responses in a timely manner through the degradation of NF-κB subunits. Furthermore, WSB1 exhibits broad tissue distribution, with higher expression levels in the spleen and lungs, and its dysfunction has been associated with various disease processes, including tumor proliferation and metastasis as well as neurological disorders.
免疫印迹
WB result of WSB1 Recombinant Rabbit mAb
Primary antibody: WSB1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293F transfected with empty vector whole cell lysate 20 µg
Lane 2: 293F transfected with WSB1-Myc-His fusion protein whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 42 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-WSB1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-WSB1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-WSB1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-WSB1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostatic cancer. Anti-WSB1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of WSB1 in tumor tissues.
Expression of WSB1 in human tissues.
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