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宿主来源
Rabbit抗原名称
VEGF Receptor 2分子别名
Vascular endothelial growth factor receptor 2; VEGFR-2; Fetal liver kinase 1 (FLK-1); Kinase insert domain receptor (KDR); Protein-tyrosine kinase receptor flk-1; CD309; FLK1; VEGFR2; KDR免疫原
Synthetic Peptide细胞定位
Cell membrane, Endoplasmic reticulumAccession
P35968克隆号
S-3912-20抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HUVEC, mouse brain, mouse heart, rat heart纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20°C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt
VEGF Receptor 2 (VEGFR-2), also known as KDR in humans and Flk-1 in mice, is a crucial receptor tyrosine kinase that plays a central role in angiogenesis, the formation of new blood vessels. This receptor has high affinity for VEGF-A and mediates most of the endothelial growth, migration, and survival signals induced by VEGF-A. Structurally, VEGFR-2 is a glycosylated transmembrane protein with a molecular weight of about 230 kDa, consisting of an extracellular domain (ECD) for ligand binding, a transmembrane domain (TMD), a juxtamembrane domain (JMD), a tyrosine kinase domain (TKD), and a C-terminal domain (CTD) for signaling. Upon binding to VEGF-A, VEGFR-2 undergoes dimerization and autophosphorylation, activating downstream signaling pathways such as the PLCγ-PKC-MAPK pathway, which promotes endothelial cell proliferation, migration, and vascular permeability. VEGFR-2 is widely expressed in vascular endothelial cells and is also found in some tumor cells, making it a key target for both pro-angiogenic therapies in conditions like ischemic diseases and anti-angiogenic therapies in cancer.
免疫印迹
WB result of VEGF Receptor 2 Recombinant Rabbit mAb
Primary antibody: VEGF Receptor 2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HUVEC whole cell lysate 20 µg
Negative control: HeLa whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 152 kDa
Observed MW: 250, 280 kDa
This blot was developed with high sensitivity substrateWB result of VEGF Receptor 2 Recombinant Rabbit mAb
Primary antibody: VEGF Receptor 2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Lane 2: mouse heart lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 152 kDa
Observed MW: 280 kDa
This blot was developed with high sensitivity substrateWB result of VEGF Receptor 2 Recombinant Rabbit mAb
Primary antibody: VEGF Receptor 2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat heart lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 152 kDa
Observed MW: 210, 280 kDa
This blot was developed with high sensitivity substrate
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