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宿主来源
Rabbit抗原名称
TRFE分子别名
Serotransferrin; Beta-1 metal-binding globulin; Siderophilin; Trf; Tf免疫原
Synthetic Peptide细胞定位
SecretedAccession
Q921I1抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Ms, Rt阳性样本
Mouse liver, mouse kidney, mouse testis, rat liver, rat kidney, rat testis纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Ms, Rt IHC-P 1:200 Ms, Rt
Transferrin receptor (TRFE), more commonly referred to as the transferrin receptor 1 (TfR1 or CD71), is a type II transmembrane glycoprotein that plays a pivotal role in cellular iron homeostasis by mediating the endocytic uptake of iron-bound transferrin from the bloodstream. Primarily expressed on the surface of erythroid precursors and other rapidly dividing cells, TRFE functions as a homodimer that binds diferric transferrin with high affinity at neutral pH, triggering receptor-mediated endocytosis; upon internalization into acidic endosomes, the drop in pH causes the release of iron from transferrin, which is then transported into the cytoplasm via divalent metal transporter 1 (DMT1) for essential processes such as hemoglobin synthesis and DNA replication, while the apotransferrin-receptor complex recycles back to the cell surface. Due to its critical involvement in iron acquisition, TRFE expression is tightly regulated by intracellular iron levels through the iron-responsive element/iron-regulatory protein (IRE/IRP) system, making it not only a fundamental component of physiological iron metabolism but also a significant biomarker for proliferative activity and a potential therapeutic target in conditions ranging from iron-deficiency anemia to various malignancies where iron demand is heightened.
免疫印迹
WB result of TRFE Rabbit pAb
Primary antibody: TRFE Rabbit pAb at 1/1000 dilution
Lane 1: mouse liver lysate 20 µg
Lane 2: mouse kidney lysate 20 µg
Lane 3: mouse testis lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 77 kDa
Observed MW: 77 kDaWB result of TRFE Rabbit pAb
Primary antibody: TRFE Rabbit pAb at 1/1000 dilution
Lane 1: rat liver lysate 20 µg
Lane 2: rat kidney lysate 20 µg
Lane 3: rat testis lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 77 kDa
Observed MW: 77 kDa
免疫组化
IHC shows positive staining in paraffin-embedded mouse liver. Anti-TRFE antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-TRFE antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-TRFE antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of TRFE in mouse & rat tissues.
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