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Phospho-IκBα (Ser32) Recombinant Rabbit mAb (S-1084-395)

NF-kappa-B inhibitor alpha,I-kappa-B-alpha (IkB-alpha,IkappaBalpha),Major histocompatibility complex enhancer-binding protein MAD3,IKBA,MAD3,NFKBI,NFKBIA

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B6000
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产品介绍

产品介绍 FAQs 引用文献(1) 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Phospho-IκBα (Ser32)

  • 分子别名

    NF-kappa-B inhibitor alpha; I-kappa-B-alpha (IkB-alpha; IkappaBalpha); Major histocompatibility complex enhancer-binding protein MAD3; IKBA; MAD3; NFKBI; NFKBIA

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Nucleus, Cytoplasm

  • Accession

    P25963

  • 克隆号

    S-1084-395

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 翻译后修饰类型

    磷酸化

  • 反应种属 ?

    Hu, Ms

  • 预测反应种属
    (反应种属缩写表)

    Pg

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    WB

  • 稀释度

    应用 稀释度 推荐种属
    Dot Blot 1:1000
    WB 1:1000 Hu, Ms
背景介绍

  • Phospho-IκBα (Ser32) is a phosphorylated form of the IκBα protein, which plays a crucial role in the NF-κB signaling pathway. IκBα normally inhibits NF-κB by binding to it and preventing its nuclear translocation. However, when IκBα is phosphorylated at Ser32 (and often also at Ser36), it undergoes ubiquitination and subsequent degradation by the 26S proteasome, thereby releasing NF-κB to translocate to the nucleus and activate gene expression. This phosphorylation event is typically mediated by the IKK complex in response to various stimuli such as cytokines, growth factors, and stress signals. Antibodies specific to Phospho-IκBα (Ser32) are widely used in research to detect and quantify this phosphorylated protein, helping to elucidate the activation status of the NF-κB pathway in different cellular contexts.

  • 免疫印迹

    • WB result of Phospho-IκBα (Ser32) Recombinant Rabbit mAb
      Primary antibody: Phospho-IκBα (Ser32) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated HeLa whole cell lysate 20 µg
      Lane 2: HeLa treated with 100 ng/ml Calyculin A for 30 minutes and 20 ng/ml TNF-α for 5 minutes whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 36 kDa
      Observed MW: 38 kDa

    • WB result of Phospho-IκBα (Ser32) Recombinant Rabbit mAb
      Primary antibody: Phospho-IκBα (Ser32) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
      Lane 2: NIH/3T3 treated with 20 ng/ml TNF-α for 10 minutes and 100 nM Calyculin A for 10 minutes whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 36 kDa
      Observed MW: 38 kDa
      This blot was developed with high sensitivity substrate

  • 斑点杂交

    • Dot blot result of Phospho-IκBα (Ser32) Recombinant Rabbit mAb
      Lane 1: IκBα (Ser32) phospho peptide
      Lane 2: IκBα (Ser32) unmodified peptide
      Primary antibody: Phospho-IκBα (Ser32) Recombinant Rabbit mAb at 1/1000 dilution
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

检测磷酸化蛋白时，推荐有什么封闭体系？

我们推荐客户使用TPST+5%脱脂奶粉来稀释一抗，进行封闭。 虽然BSA被推荐为WB检测磷酸化蛋白的常用封闭剂，但是脱脂奶粉获取更加方便，覆盖更广泛的非特异性结合位点，在一抗性能优越的前提下，使用脱脂奶粉封闭性价比更高

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