RBBP4 Rabbit Polyclonal Antibody
Histone-binding protein RBBP4,Chromatin assembly factor 1 subunit C (CAF-1 subunit C),Chromatin assembly factor I p48 subunit (CAF-I 48 kDa subunit,CAF-I p48),Nucleosome-remodeling factor subunit RBAP48,Retinoblastoma-binding protein 4 (RBBP-4),Retinoblastoma-binding protein p48,RBAP48
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宿主来源
Rabbit抗原名称
RBBP4分子别名
Histone-binding protein RBBP4; Chromatin assembly factor 1 subunit C (CAF-1 subunit C); Chromatin assembly factor I p48 subunit (CAF-I 48 kDa subunit; CAF-I p48); Nucleosome-remodeling factor subunit RBAP48; Retinoblastoma-binding protein 4 (RBBP-4); Retinoblastoma-binding protein p48; RBAP48免疫原
Synthetic Peptide细胞定位
NucleusAccession
Q09028抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
SH-SY5Y, HeLa Jurkat, HEK-293, mouse spleen, F9, rat spleen纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Hu, Ms, Rt IHC-P 1:1000 Hu, Ms, Rt
RBBP4 (Retinoblastoma Binding Protein 4), also known as RbAp48 or NURF55, is a highly conserved core subunit of several essential chromatin-modifying complexes, including the NuRD (Nucleosome Remodeling and Deacetylase) complex, the PRC2 (Polycomb Repressive Complex 2), and the HAT1 histone acetyltransferase complex. Functioning primarily as a histone chaperone, RBBP4 facilitates the assembly and stability of nucleosomes by binding directly to the N-terminal tails of histones H3 and H4, thereby playing a pivotal role in regulating chromatin structure and epigenetic gene expression. Through its involvement in these multiprotein complexes, RBBP4 contributes to critical cellular processes such as transcriptional repression, DNA replication, cell cycle progression, and cellular differentiation, while its dysregulation has been implicated in various pathological conditions, including cancer and neurodegenerative disorders, highlighting its significance as both a fundamental regulator of genome integrity and a potential therapeutic target.
免疫印迹
WB result of RBBP4 Rabbit pAb
Primary antibody: RBBP4 Rabbit pAb at 1/1000 dilution
Lane 1: SH-SY5Y whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: HEK-293 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 50 kDaWB result of RBBP4 Rabbit pAb
Primary antibody: RBBP4 Rabbit pAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Lane 2: mouse spleen lysate 20 µg
Lane 3: F9 whole cell lysate 20 µg
Low expression control: mouse brain lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 50 kDaWB result of RBBP4 Rabbit pAb
Primary antibody: RBBP4 Rabbit pAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Lane 2: rat spleen lysate 20 µg
Low expression control: rat brain lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 50 kDaWB result of RBBP4 Rabbit pAb
Primary antibody: RBBP4 Rabbit pAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 50 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-RBBP4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-RBBP4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-RBBP4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-RBBP4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-RBBP4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-RBBP4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-RBBP4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of RBBP4 in tumor tissues.
Expression of RBBP4 in human tissues.
Expression of RBBP4 in mouse & rat tissues.
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