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Mouse抗原名称
XCR1分子别名
Chemokine XC receptor 1; Lymphotactin receptor; SCM1 receptor; XC chemokine receptor 1; mXCR1; Ccxcr1; Xcr1细胞定位
Cell membraneAccession
Q9R0M1克隆号
S-R601抗体类型
Mouse mAb抗体同种型
IgG2b,k反应种属 ?
Ms阳性样本
collagenase-digested C57BL/6 mouse splenocytes纯化方式
Protein A浓度
0.2 mg/ml标记
Biotin性状
Liquid缓冲体系
PBS pH7.4, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Ms
XCR1 is a conserved, 37-kDa, seven-transmembrane, G-protein-coupled receptor selectively expressed on CD8α⁺ and CD103⁺ conventional dendritic cells; its only ligand, XCL1, is secreted by activated CD8⁺ T and NK cells to form a feed-forward loop that efficiently recruits antigen-bearing dendritic cells into draining lymph nodes, licenses cross-presentation, and amplifies cytotoxic immune responses against viruses and tumors, while genetic or antibody blockade of XCR1 impairs viral clearance, vaccine efficacy, and checkpoint-blockade-mediated anti-tumor immunity.
流式分析
Flow cytometric analysis of Mouse XCR1 expression on collagenase-digested C57BL/6 mouse splenocytes. Collagenase-digested C57BL/6 mouse splenocytes were stained with Alexa Fluor® 647 Rat Anti-Mouse CD8 antibody and either Biotin Mouse IgG Isotype Control (Left panel) or SDT Biotin Mouse Anti- Mouse XCR1 antibody (Right panel) at 5 μl/test followed by Sav-iFluor 488. Data shown was gated on the CD11c+ population. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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