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宿主来源
Mouse抗原名称
CD15分子别名
Sialyl LewisX (sLeX); SSEA1克隆号
HI98抗体类型
Mouse mAb抗体同种型
IgM反应种属 ?
Hu阳性样本
Human peripheral blood Leukocytes纯化方式
Protein G浓度
0.2 mg/ml标记
Alexa Fluor® 488性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1.25μl per million cells in 100μl volume Hu
CD15, also known as Lewis X or SSEA-1, is a carbohydrate epitope (Galβ1→4[Fucα1→3]GlcNAc) carried primarily on glycoproteins and glycolipids of myeloid cells, granulocytes, and certain lymphocyte subsets; it serves as a key adhesion molecule that mediates selectin-dependent leukocyte rolling during inflammation, acts as a marker of mature neutrophils and Hodgkin/Reed-Sternberg cells, and its differential expression is exploited in immunohistochemistry to distinguish myeloid leukemias from lymphoid malignancies and to identify stem-cell populations in normal and neoplastic tissues.
流式分析
Flow cytometric analysis of Human CD15 expression on human peripheral blood Leukocytes. Human peripheral blood Leukocytes were stained with Alexa Fluor® 488 Mouse IgM Isotype Control (Left panel) or SDT Alexa Fluor® 488 Mouse Anti-Human CD15 Antibody (Right panel) at 1.25 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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