分享产品介绍 评论(0)
宿主来源
Rat抗原名称
Ly-6C分子别名
Lymphocyte antigen 6C1; Ly-6C1; Ly6c1细胞定位
Cell membraneAccession
P0CW02克隆号
HK1.4抗体类型
Rat mAb抗体同种型
IgG2c,k反应种属 ?
Ms阳性样本
C57BL/6 mouse bone marrow纯化方式
Protein G浓度
0.2 mg/ml标记
APC-Cy7性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Ms
Ly-6C is a glycosylphosphatidylinositol-anchored cell-surface protein belonging to the Ly-6/uPAR/α-neurotoxin superfamily, selectively expressed on murine monocyte and T-cell subsets where it serves as a dynamic lineage and activation marker—its high level on pro-inflammatory “classical” monocytes (CD11b⁺Ly-6C^hi) enables their recruitment via CCR2 to inflamed tissues, whereas Ly-6C^lo monocytes patrol vasculature and replenish resident macrophages, and transient up-regulation on CD8⁺ T cells correlates with effector differentiation; structurally it forms a three-finger α-helical bundle that can laterally associate with lipid rafts and signaling partners such as T-cell receptor or integrin complexes to modulate calcium flux, cytokine release, and adhesion, thereby critically steering innate–adaptive immune crosstalk in infections, autoimmunity, and cardiovascular disease.
流式分析
Flow cytometric analysis of Mouse Ly-6C expression on C57BL/6 mouse bone marrow. C57BL/6 mouse bone marrow cells were stained with Brilliant Violet 510™ Rat Anti-Mouse CD11b and either APC-Cy7 Rat IgG2c, κ Isotype Control (Left panel) or APC-Cy7 Rat Anti-Mouse Ly-6C Antibody (Right panel) at 5 μl/test treated with True-Stain Monocyte Blocker™. Total viable cells, as determined by Fixable Viability Dye 515 (S0D0013), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
评论(0)