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宿主来源
Mouse抗原名称
CD158b/j分子别名
CD158b1; KIR2DL2; CD158B1; NKAT2a; NKAT2b; CD158b2; KIR2DL3; CD158B2; KIRCL23; NKAT2; CD158j; KIR2DS2; CD158J; NKAT5细胞定位
Cell membraneAccession
P43627, P43628, P43631克隆号
S-2917抗体类型
Mouse mAb抗体同种型
IgG2a,k反应种属 ?
Hu阳性样本
Human peripheral blood Leukocytes纯化方式
Protein A浓度
0.2 mg/ml标记
APC性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Hu
CD158b/j is a collective designation for the killer-cell immunoglobulin-like receptors (KIR2DL2, KIR2DL3 and KIR2DS2), 50–58 kDa type-I transmembrane glycoproteins expressed chiefly on natural killer cells and minor T-cell subsets; each molecule carries two extracellular Ig-like C2 domains that recognize distinct HLA-C alleles (HLA-Cw1/3/7 for the inhibitory forms), and while the long-tailed CD158b variants (KIR2DL2/L3) deliver inhibitory signals via dual cytoplasmic ITIM motifs to prevent target-cell lysis, the short-tailed CD158j variant (KIR2DS2) can trigger activation, thereby collectively fine-tuning innate cytotoxic responses and influencing immune escape in infection, transplantation and cancer.
流式分析
Flow cytometric analysis of Human CD158b expression on human peripheral blood Leukocytes. Human peripheral blood Leukocytes were stained with PE Mouse Anti-Human CD56 Antibody and either APC Mouse IgG2a, κ Isotype Control (Left panel) or SDT APC Mouse Anti-Human CD158b/j Antibody (Right panel) at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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