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宿主来源
Mouse抗原名称
CD3分子别名
T-cell surface glycoprotein CD3 gamma chain; T-cell receptor T3 gamma chain; Cd3g细胞定位
Cell membraneAccession
Q64159, P19377克隆号
1F4抗体类型
Mouse mAb抗体同种型
IgG2b反应种属 ?
Rt阳性样本
Wistar Rat splenocytes纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1:200 Rt
CD3 is a multimeric protein complex that serves as a crucial T-cell co-receptor, composed of four distinct chains (CD3γ, δ, ε, ζ) that non-covalently associate with the T-cell receptor (TCR), where its cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs) become phosphorylated upon antigen recognition to initiate downstream signaling cascades leading to T-cell activation, differentiation, and effector functions, making it an essential component of adaptive immunity and a valuable target for therapeutic interventions such as the immunosuppressive agent muromonab-CD3 and emerging bispecific antibodies in oncology.
流式分析
Flow cytometric analysis of Wistar Rat splenocytes labelling CD3 antibody at 1/200 (1 μg) dilution (Right panel) compared with a Mouse IgG2b Isotype Control (Left panel). Goat Anti-Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD45R/B220 - PE antibody separately.
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