分享产品介绍 评论(0)
宿主来源
Armenian hamster抗原名称
CD30分子别名
Tumor necrosis factor receptor superfamily member 8; CD30L receptor; Lymphocyte activation antigen CD30; Tnfrsf8细胞定位
Cell membraneAccession
Q60846克隆号
mCD30.1抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Ms阳性样本
ConA-treated C57BL/6 mouse splenocytes纯化方式
Protein G浓度
0.2 mg/ml标记
Alexa Fluor® 647性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1.25μl per million cells in 100μl volume Ms
CD30 (TNFRSF8) is a 120 kDa type-I transmembrane glycoprotein of the tumor necrosis factor receptor superfamily, expressed on activated T and B cells, Reed–Sternberg cells of classical Hodgkin lymphoma, and anaplastic large-cell lymphoma, where it regulates proliferation, apoptosis, and NF-κB signaling through TRAF1/2/5 adaptor proteins upon trimerization with its ligand CD30L (CD153), and serves as the validated target for the antibody–drug conjugate brentuximab vedotin that delivers the antimicrotubule agent monomethyl auristatin E into CD30-positive malignant cells.
流式分析
Flow cytometric analysis of Mouse CD30 expression on ConA-treated C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes treated 72h with ConA were stained with either Alexa Fluor® 647 Armenian hamster IgG Isotype Control (Left panel) or Alexa Fluor® 647 Armenian hamster Anti-Mouse CD30 Antibody (Right panel) at 1.25 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
评论(0)