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宿主来源
Mouse抗原名称
CD28分子别名
T-cell-specific surface glycoprotein CD28; TP44; CD28细胞定位
Cell membraneAccession
P10747克隆号
9.3抗体类型
Mouse mAb抗体同种型
IgG1反应种属 ?
Hu阳性样本
human PBMC纯化方式
Protein G浓度
0.2 mg/ml标记
PE性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1.25μl per million cells in 100μl volume Hu
CD28, also known as the T-cell-specific surface glycoprotein CD28, is a protein encoded by the CD28 gene in humans. It is a glycoprotein that is constitutively expressed on both resting and activated T cells. CD28 plays a crucial role in T cell activation and signaling, providing essential co-stimulatory signals required for T cell activation, proliferation, and cytokine production. It enhances the production of IL4 and IL10 in T cells in conjunction with TCR/CD3 ligation and CD40L co-stimulation. CD28 interacts with its ligands, CD80 (B7-1) and CD86 (B7-2), which are expressed on antigen-presenting cells, to mediate the co-stimulatory signals necessary for T cell activation. This interaction is critical for the early events of T cell activation and the decision between immunity and anergy.
流式分析
Flow cytometric analysis of Human CD28 expression on human PBMC (human peripheral blood mononuclear cell). Human PBMC were stained with Brilliant Violet 421™ Mouse Anti-Human CD3 antibody and either PE Mouse IgG1 Isotype Control (Left panel) or SDT PE Mouse Anti-Human CD28 Antibody (Right panel) at 1.25 μl/test. Total viable cells, as determined by Fixable Viability Dye 662 (S0D0016), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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