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宿主来源
Mouse抗原名称
Granzyme B分子别名
C11; CTLA-1; Cathepsin G-like 1 (CTSGL1); Cytotoxic T-lymphocyte proteinase 2 (Lymphocyte protease); Fragmentin-2; CGL1; CSPB; CTLA1; GRB; GZMB细胞定位
SecretedAccession
P10144, P04187克隆号
S-2865抗体类型
Mouse mAb抗体同种型
IgG1,k反应种属 ?
Hu, Ms阳性样本
Human PBMC纯化方式
Protein G浓度
0.2 mg/ml标记
Alexa Fluor® 488性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
ICFCM
稀释度
应用 稀释度 推荐种属 ICFCM 5μl per million cells in 100μl volume Hu, Ms
Granzyme B is a 32~kDa serine protease stored in the cytolytic granules of cytotoxic T lymphocytes and natural killer cells, whose immune function centers on perforin-assisted translocation into target cells where it directly cleaves and activates pro-apoptotic substrates such as procaspase-3, Bid and PARP, thereby igniting both caspase-dependent and caspase-independent mitochondrial apoptosis pathways to eliminate virus-infected or malignant cells.
流式分析
Flow cytometric analysis of Human Granzyme B expression in Human PBMC. Human PBMC were fixed with 4% PFA and permeabilized with 0.1% Tween. The cells were stained with Alexa Fluor® 647 Mouse Anti-Human CD3 Antibody and either Alexa Fluor®488 Mouse IgG1, κ Isotype Control (Left panel) or Alexa Fluor®488 Mouse Anti-Human/Mouse Granzyme B Antibody (Right panel) at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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