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宿主来源
Mouse抗原名称
CD144分子别名
Cadherin-5; 7B4 antigen; Vascular endothelial cadherin (VE-cadherin); CDH5细胞定位
Cell membrane, Cell junctionAccession
P33151克隆号
S-3026抗体类型
Mouse mAb抗体同种型
IgG1,k反应种属 ?
Hu阳性样本
HUVEC纯化方式
Protein G浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1:400 Hu
CD144, also termed VE-cadherin or cadherin-5, is a 140 kDa calcium-dependent type-II classical cadherin expressed almost exclusively on vascular and lymphatic endothelial cells, where it forms the core of adherens junctions by mediating homophilic trans-interactions between neighboring cells and anchoring to the actin cytoskeleton via catenins (β-, γ-, and p120-catenin), thereby controlling endothelial barrier integrity, vascular permeability, leukocyte extravasation, and embryonic angiogenesis, while also modulating VEGF receptor signaling, cell polarity, and lumen formation .
流式分析
Flow cytometric analysis of HUVEC (Human umbilical vein endothelial cell) labelling CD144 antibody at 1/400 dilution (0.5 μg) / (Red) compared with a Mouse IgG1, κ Isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 647 was used as the secondary antibody.
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