分享产品介绍 评论(0)
宿主来源
Mouse抗原名称
CD199分子别名
C-C chemokine receptor type 9; C-C CKR-9; CC-CKR-9; CCR-9; G-protein coupled receptor 28; GPR-9-6; CDw199; GPR28; CCR9细胞定位
Cell membraneAccession
P51686克隆号
S-3012抗体类型
Mouse mAb抗体同种型
IgG2a,k反应种属 ?
Hu阳性样本
MOLT-4纯化方式
Protein A浓度
0.2 mg/ml标记
Alexa Fluor® 488性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Hu
CD199 (also designated CCR9) is a ~42 kDa seven-transmembrane G-protein-coupled chemokine receptor that binds the chemokine CCL25 (TECK), is strongly expressed in thymic cortex and small intestinal epithelium, and is highly expressed on CD4+CD8+ double-positive thymocytes and naive CD8+ T cells, as well as on small intestinal B cells, subsets of memory CD4+/CD8+ T cells, and dendritic cells, thereby directing T-cell development in the thymus and lymphocyte homing to gut-associated tissues via the CCR9/CCL25 axis, with dysregulation implicated in inflammatory bowel disease and celiac disease .
流式分析
Flow cytometric analysis of Human CD199 expression on MOLT-4 cells. Cells from the MOLT-4 (Human lymphoblastic leukemia T lymphoblast, Right) or Raji (Human Burkitt's lymphoma B lymphocyte, Left) was stained with either Alexa Fluor® 488 Mouse IgG2a, κ Isotype Control (Black line histogram) or SDT Alexa Fluor® 488 Mouse Anti-Human CD199 Antibody (Red line histogram) at 5 μl/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
评论(0)