Alexa Fluor® 647 Mouse Anti-Human CD140a Antibody (S-2840)
Platelet-derived growth factor receptor alpha,PDGF-R-alpha,PDGFR-alpha,Alpha platelet-derived growth factor receptor,Alpha-type platelet-derived growth factor receptor,CD140 antigen-like family member A,CD140a antigen,Platelet-derived growth factor alpha receptor,Platelet-derived growth factor receptor 2 (PDGFR-2),CD140a,PDGFR2,RHEPDGFRA,PDGFRA
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宿主来源
Mouse抗原名称
CD140a分子别名
Platelet-derived growth factor receptor alpha; PDGF-R-alpha; PDGFR-alpha; Alpha platelet-derived growth factor receptor; Alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a antigen; Platelet-derived growth factor alpha receptor; Platelet-derived growth factor receptor 2 (PDGFR-2); CD140a; PDGFR2; RHEPDGFRA; PDGFRA细胞定位
Cell membraneAccession
P16234克隆号
S-2840抗体类型
Mouse mAb抗体同种型
IgG2a,k反应种属 ?
Hu阳性样本
A204纯化方式
Protein A浓度
0.2 mg/ml标记
Alexa Fluor® 647性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1.25μl per million cells in 100μl volume Hu
CD140a (also called platelet-derived growth factor receptor-alpha, PDGFR-α) is a 170-kDa glycosylated cell-surface receptor tyrosine kinase encoded by the PDGFRA gene, featuring an extracellular ligand-binding region with five immunoglobulin-like domains, a single transmembrane segment and an intracellular split tyrosine-kinase domain; upon binding PDGF-AA, -AB or -BB it forms homo- or heterodimers, triggers downstream signalling cascades that drive proliferation, migration and differentiation of mesenchymal and glial cells, and is indispensable for embryogenesis of kidney, testis and other organs, wound healing and maintenance of hematologic tissues, while PDGFRA mutations or amplifications are linked to clonal hypereosinophilic neoplasms, gastrointestinal stromal tumours, gliomas and hepatocellular carcinoma, making CD140a a potential therapeutic target in these cancers .
流式分析
Flow cytometric analysis of Human CD140a expression on A204 cells. Cells from the A204 (Human rhabdomyosarcoma cells, Right) or U87-MG (Human glioblastoma-astrocytoma epithelial cell, Left) was stained with either Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control (Black line histogram) or SDT Alexa Fluor® 647 Mouse Anti-Human CD140a Antibody (Red line histogram) at 1.25 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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