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宿主来源
Mouse抗原名称
CD235a/b分子别名
Glycophorin-A, MN sialoglycoprotein, PAS-2, Sialoglycoprotein alpha, GYPA, Glycophorin-B, PAS-3, SS-active sialoglycoprotein, Sialoglycoprotein delta, GYPB细胞定位
Cell membraneAccession
P02724, P06028克隆号
HIR2抗体类型
Mouse mAb抗体同种型
IgG2b,k反应种属 ?
Hu阳性样本
human peripheral blood纯化方式
Protein A浓度
0.2 mg/ml标记
PE性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1.25μl per million cells in 100μl volume Hu
Glycophorin A (GYPA) and B (GYPB; this protein) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups respectively. In addition to the M or N and S or s antigens, that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include the Miltenberger (Mi) complex and several isoforms of Stones (Sta); also Dantu, Sat, Henshaw (He or MNS6), Mg and deletion variants Ena, S-s-U- and Mk. Most of these are the result of gene recombinations between GYPA and GYPB.
流式分析
Flow cytometric analysis of Human CD235a/b expression on human peripheral blood. human peripheral blood cells were stained with V500 Mouse Anti-Human CD45 Antibody and either PE Mouse IgG2b, κ Isotype Control (Left panel) or SDT PE Mouse Anti-Human CD235a/b Antibody (Right panel) at 1.25 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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