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宿主来源
Mouse抗原名称
IL-8分子别名
Interleukin-8; IL-8; C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8; Emoctakin; Granulocyte chemotactic protein 1 (GCP-1); Monocyte-derived neutrophil chemotactic factor (MDNCF); IL8; CXCL8细胞定位
SecretedAccession
P10145克隆号
S-2875抗体类型
Mouse mAb抗体同种型
IgG2b反应种属 ?
Hu纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
ICFCM
稀释度
应用 稀释度 推荐种属 ICFCM 1:2000 Hu
Interleukin-8 (IL-8), also known as CXCL8, is a small 8–10 kDa chemokine produced by monocytes, macrophages, endothelial cells, and epithelial cells in response to inflammatory stimuli such as TNF-α, IL-1β, or bacterial LPS; it acts primarily through the G-protein-coupled receptors CXCR1 and CXCR2 on neutrophils to induce rapid chemotaxis, degranulation, and respiratory burst, thereby orchestrating acute inflammatory responses, angiogenesis, and tumor microenvironment remodeling, while dysregulated IL-8 expression is implicated in chronic inflammatory diseases, COPD, atherosclerosis, cancer progression, and metastasis.
流式分析
Flow cytometric analysis of 10ng/ml lipopolysaccharide and 1μg/ml BFA cultured human PBMC (human peripheral blood mononuclear cell) labelling human IL-8 antibody at 1/2000 dilution (0.1 μg) / (Right Panel) compared with a Mouse IgG2b Isotype control (Left Panel). Goat Anti - Mouse IgG Alexa Fluor® 647 was used as the secondary antibody. Then cells were stained with CD14 - Brilliant Violet 510™ separately. Gated on total viable cells.
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