APC Rat Anti-Mouse F4/80 Antibody (S-R537)
Adhesion G protein-coupled receptor E1,Cell surface glycoprotein F4/80,EGF-like module receptor 1,EGF-like module-containing mucin-like hormone receptor-like 1,EMR1 hormone receptor,Emr1,Gpf480,Adgre1
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宿主来源
Rat抗原名称
F4/80分子别名
Adhesion G protein-coupled receptor E1; Cell surface glycoprotein F4/80; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EMR1 hormone receptor; Emr1; Gpf480; Adgre1细胞定位
Cell membraneAccession
Q61549克隆号
S-R537抗体类型
Rat mAb抗体同种型
IgG2a,k反应种属 ?
Ms阳性样本
RAW264.7纯化方式
Protein G浓度
0.2 mg/ml标记
APC性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1.25μl per million cells in 100μl volume Ms
F4/80, also known as ADGRE1 or EMR1, is a cell surface glycoprotein and a member of the EGF-TM7 protein family. It is widely used as a marker for mature mouse macrophages and is highly expressed in various macrophage populations, such as Kupffer cells in the liver, Langerhans cells in the skin, and microglial cells in the brain. However, its expression in human cells is different, being mainly found in eosinophils and some macrophages. F4/80 is an orphan receptor involved in cell adhesion and immune cell interactions, and it may also play a role in the development of regulatory T cells. The protein has a large extracellular domain containing multiple EGF-like calcium-binding domains, linked to a seven-transmembrane domain characteristic of G protein-coupled receptors.
流式分析
Flow cytometric analysis of Mouse F4/80 expression on RAW264.7 cells. Cells from the RAW264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage, Right) or C2C12 (Mouse myoblasts myoblast, Left) was stained with either APC Rat IgG2a, κ Isotype Control (Black line histogram) or SDT APC Rat Anti-Mouse F4/80 Antibody (Red line histogram) at 1.25 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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