Alexa Fluor® 647 Mouse Anti-Human IL-8 Antibody (S-2875)
Interleukin-8,IL-8,C-X-C motif chemokine 8,Chemokine (C-X-C motif) ligand 8,Emoctakin,Granulocyte chemotactic protein 1 (GCP-1),Monocyte-derived neutrophil chemotactic factor (MDNCF),IL8,CXCL8
分享产品介绍 评论(0)
宿主来源
Mouse抗原名称
IL-8分子别名
Interleukin-8; IL-8; C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8; Emoctakin; Granulocyte chemotactic protein 1 (GCP-1); Monocyte-derived neutrophil chemotactic factor (MDNCF); IL8; CXCL8细胞定位
SecretedAccession
P10145克隆号
S-2875抗体类型
Mouse mAb抗体同种型
IgG2b反应种属 ?
Hu纯化方式
Protein A浓度
0.2 mg/ml标记
Alexa Fluor® 647性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
ICFCM
稀释度
应用 稀释度 推荐种属 ICFCM 1.25μl per million cells in 100μl volume Hu
Interleukin-8 (IL-8), also known as CXCL8, is a small 8–10 kDa chemokine produced by monocytes, macrophages, endothelial cells, and epithelial cells in response to inflammatory stimuli such as TNF-α, IL-1β, or bacterial LPS; it acts primarily through the G-protein-coupled receptors CXCR1 and CXCR2 on neutrophils to induce rapid chemotaxis, degranulation, and respiratory burst, thereby orchestrating acute inflammatory responses, angiogenesis, and tumor microenvironment remodeling, while dysregulated IL-8 expression is implicated in chronic inflammatory diseases, COPD, atherosclerosis, cancer progression, and metastasis.
流式分析
Flow cytometric analysis of IL-8 expression in stimulated Human PBMC (human peripheral blood mononuclear cells). Human PBMC were cultured 6 hr with 10ng/ml lipopolysaccharide in the presence of 1μg/ml BFA. The PBMC were harvested and fixed with 4% PFA and permeabilized with Intracellular Fixation & Permeabilization Buffer Set. The cells were then stained with Brilliant Violet 510™ Mouse Anti-Human CD14 and either Mouse IgG2b Isotype Control or SDT Alexa Fluor® 647 Mouse Anti-Human IL-8 Antibody at 1.25μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
评论(0)