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宿主来源
Mouse抗原名称
CD34分子别名
Hematopoietic progenitor cell antigen CD34细胞定位
Cell membraneAccession
P28906克隆号
4H11抗体类型
Mouse mAb抗体同种型
IgG1,k反应种属 ?
Hu阳性样本
human PBMC纯化方式
Protein G标记
PE性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 10μl per million cells in 100μl volume Hu
CD34 is a transmembrane phosphoglycoprotein protein encoded by the CD34 gene. CD34 derives its name from the cluster of differentiation protocol that identifies cell surface antigens. CD34 was first described on hematopoietic stem cells independently by Civin et al. and Tindle et al, as a cell surface glycoprotein and functions as a cell-cell adhesion factor. It may also mediate the attachment of hematopoietic stem cells to bone marrow extracellular matrix or directly to stromal cells. Clinically, it is associated with the selection and enrichment of hematopoietic stem cells for bone marrow transplants. Due to these historical and clinical associations, CD34 expression is almost ubiquitously related to hematopoietic cells.
流式分析
Flow cytometric analysis of Human CD34 expression on human PBMC (human peripheral blood mononuclear cell). Human PBMC were stained with FITC Mouse Anti-Human Lineage Cocktail antibody and either PE Mouse IgG1, κ Isotype Control (Left panel) or SDT PE Mouse Anti-Human CD34 Antibody (Right panel) at 10 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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