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宿主来源
Mouse抗原名称
CD9分子别名
CD9 antigen; 5H9 antigen; Cell growth-inhibiting gene 2 protein; Leukocyte antigen MIC3; Motility-related protein (MRP-1); Tetraspanin-29 (Tspan-29); p24; MIC3; TSPAN29细胞定位
Cell membraneAccession
P21926克隆号
S-2896抗体类型
Mouse mAb抗体同种型
IgG1,k反应种属 ?
Hu阳性样本
human peripheral blood platelets纯化方式
Protein G浓度
0.2 mg/ml标记
Alexa Fluor® 647性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Hu
CD9, a 24-kDa cell-surface glycoprotein belonging to the tetraspanin family, is characterized by four transmembrane domains and two extracellular loops; it is widely expressed (e.g., on platelets, B/T cells, macrophages, and oocytes) and organizes tetraspanin-enriched microdomains that integrate membrane and cytoplasmic proteins to regulate adhesion, migration, proliferation, survival, and immune synapse formation. Notably, CD9 is essential for mammalian gamete fusion, as CD9-null eggs cannot fuse with wild-type sperm, and anti-CD9 antibodies block sperm–egg binding and fusion in vitro. Additionally, CD9 is implicated in platelet activation, signal transduction, and is frequently detected in lymphoblastic leukemia/lymphoma and some acute myeloid leukemia cells.
流式分析
Flow cytometric analysis of Human CD9 expression on human peripheral blood platelets. Human peripheral blood platelets were stained with either Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (Black line histogram) or SDT Alexa Fluor® 647 Mouse Anti-Human CD9 Antibody (Red line histogram) at 5 μl/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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