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宿主来源
Mouse抗原名称
FOXP3分子别名
Forkhead box protein P3; Scurfin; IPEX细胞定位
Cytoplasm, NucleusAccession
Q9BZS1克隆号
S-2903抗体类型
Mouse mAb抗体同种型
IgG1反应种属 ?
Hu阳性样本
Human PBMC纯化方式
Protein G浓度
0.2 mg/ml标记
Alexa Fluor® 647性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
稀释度
应用 稀释度 推荐种属 ICFCM 1.25μl per million cells in 100μl volume Hu
FOXP3 protein is a crucial transcription factor that plays a central role in the development and function of regulatory T cells (Tregs), which are essential for maintaining immune tolerance and preventing autoimmune diseases. It is predominantly expressed in Tregs and helps suppress the activity of other immune cells, thereby preventing excessive immune responses. Mutations or dysfunction in the FOXP3 gene can lead to severe autoimmune disorders such as IPEX syndrome, highlighting its importance in immune regulation. Additionally, FOXP3 has been implicated in various other biological processes, including cancer immunology, where its expression in tumor-infiltrating Tregs can influence the tumor microenvironment and affect the efficacy of immunotherapies.
流式分析
Flow cytometric analysis of FOXP3 expression in PBMC (Human peripheral blood mononuclear cells). Human PBMC were fixed and permeabilized with Foxp3 / Transcription Factor Staining Buffer Set. The cells were then stained with Brilliant Violet 421™ Mouse Anti-Human CD4 Antibody and either Alexa Fluor® 647 Mouse IgG1 Isotype Control or SDT Alexa Fluor® 647 Mouse Anti-Human FOXP3 Antibody at 1.25 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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