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宿主来源
Mouse抗原名称
CD3ε分子别名
T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; Cd3e细胞定位
Cell membraneAccession
P22646克隆号
S-R384抗体类型
Mouse mAb抗体同种型
IgG1,k反应种属 ?
Ms阳性样本
C57BL/6 mouse splenocytes纯化方式
Protein G浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1:200 Ms
CD3ε protein, also known as CD3E, is a crucial component of the T-cell receptor (TCR) complex, which is essential for T-cell activation and function. It is a single-pass type I membrane glycoprotein that contains one Ig-like domain and one immunoreceptor tyrosine-based activation motif (ITAM) domain. CD3ε, along with CD3γ, CD3δ, and CD3ζ, forms the TCR-CD3 complex, which plays a vital role in coupling antigen recognition to intracellular signal-transduction pathways. This protein is essential for the proper development of T-cells and is involved in the internalization and downregulation of the TCR-CD3 complex on the cell surface. Defects in the CD3ε gene can lead to severe immunodeficiency and have been linked to a susceptibility to type I diabetes in women.
流式分析
Flow cytometric analysis of C57BL/6 mouse splenocytes labelling Mouse CD3ε antibody at 1/200 (1 μg) dilution/ (Right panel) compared with a Mouse IgG1, κ Isotype Control / (Left panel). Goat Anti-Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD19 - Alexa Fluor® 647 antibody separately.
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