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Rat抗原名称
Perforin分子别名
Perforin-1; P1; Cytolysin; Lymphocyte pore-forming protein; Pfp; Prf1细胞定位
Secreted, Cell membraneAccession
P10820克隆号
S-R644抗体类型
Rat mAb抗体同种型
IgG2a,k反应种属 ?
Ms阳性样本
BALB/c mouse splenocytes纯化方式
Protein G浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.应用
ICFCM
稀释度
应用 稀释度 推荐种属 ICFCM 1:200 Ms
Perforin is a crucial protein involved in the immune response, primarily produced by cytotoxic T lymphocytes and natural killer cells. It functions by forming pores in the membranes of target cells, such as virus-infected or cancerous cells. Once released from immune cells, perforin molecules aggregate and insert into the target cell membrane, creating channels that disrupt the cell's osmotic balance, leading to cell lysis and death. This mechanism is essential for eliminating harmful cells and maintaining the body's overall health.
流式分析
Flow cytometric analysis of Foxp3 / Transcription Factor Staining Buffer Set fixed and permeabilized BALB/c mouse splenocytes labelling Mouse Perforin antibody at 1/200 dilution (1 μg) / (Red) compared with a Rat IgG2a, κ (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rat IgG Alexa Fluor® 647 was used as the secondary antibody. Then cells were stained with CD49b - FITC antibody separately.
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