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Rat抗原名称
CD294分子别名
Prostaglandin D2 receptor 2; Chemoattractant receptor-homologous molecule expressed on Th2 cells; G-protein coupled receptor 44; CRTH2; DL1R; GPR44; PTGDR2细胞定位
Cell membraneAccession
Q9Y5Y4克隆号
S-2867抗体类型
Rat mAb抗体同种型
IgG2a,k反应种属 ?
Hu阳性样本
human PBMC纯化方式
Protein G浓度
0.2 mg/ml标记
Alexa Fluor® 647性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1.25μl per million cells in 100μl volume Hu
CD294, also known as CRTH2, PTGDR2, or GPR44, is a seven-transmembrane G protein-coupled receptor primarily expressed on Th2 cells, eosinophils, basophils, and type-2 innate lymphoid cells (ILC2). It is involved in mediating the chemotaxis of these cells to sites of allergic inflammation, such as the asthmatic lung, and plays a role in preventing apoptosis in Th2 cells.
流式分析
Flow cytometric analysis of Human CD294 expression on human PBMC (human peripheral blood mononuclear cell). Human PBMC were stained with FITC Mouse Anti-Human CD123 antibody and either Alexa Fluor® 647 Rat IgG2a, κ Isotype Control (Left panel) or SDT Alexa Fluor® 647 Rat Anti-Human CD294 Antibody (Right panel) at 1.25 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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