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宿主来源
Rat抗原名称
Ki67分子别名
Proliferation marker protein Ki-67; Antigen identified by monoclonal antibody Ki-67 homolog (Antigen KI-67 homolog; Antigen Ki67 homolog); Mki67细胞定位
NucleusAccession
E9PVX6克隆号
S-R595抗体类型
Rat mAb抗体同种型
IgG2a,k同型对照
S0B0932反应种属 ?
Ms阳性样本
NIH/3T3纯化方式
Protein G浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
ICFCM
稀释度
应用 稀释度 推荐种属 ICFCM 1:200 Ms
Ki67 protein is a non-histone nuclear protein encoded by the MKI67 gene, widely used as a proliferation marker in pathology. It is expressed during all active phases of the cell cycle—G1, S, G2, and M—but is absent in quiescent (G0) cells. Ki67 has multiple molecular functions, including forming the perichromosomal layer to prevent chromosome aggregation during mitosis and organizing heterochromatin. It also facilitates chromosome attachment to the mitotic spindle and individual chromosome mobility. In addition to its role in cell division, Ki67 is a valuable prognostic and predictive indicator in cancer diagnosis, with its expression levels correlating with tumor cell proliferation rates.
流式分析
Flow cytometric analysis of NIH/3T3 cells (Mouse embryonic fibroblast) fixed with 4% paraformaldehyde and permeabilized with 90% methanol. Cells were labeled with anti-Mouse Ki67 antibody at 1/200 dilution (1 μg) / (red) compared with a Rat IgG2a, κ (black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti - Rat IgG Alexa Fluor® 647 was used as the secondary antibody. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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