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宿主来源
Rabbit抗原名称
Glypican-3分子别名
GTR2-2; Intestinal protein OCI-5; MXR7; OCI5; GPC3细胞定位
Cell membraneAccession
P51654克隆号
SDT-R032抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
HepG2纯化方式
Protein A浓度
0.05mg/ml标记
APC性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Hu
Glypican-3 (GPC3) is a cell-surface glycoprotein that is highly expressed in hepatocellular carcinoma (HCC) and some other cancers. It is a member of the glypican family of heparan sulfate proteoglycans, consisting of a core protein and heparan sulfate chains, and is anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) anchor. GPC3 is encoded by the GPC3 gene located on the X chromosome and is cleaved by furin into an N-terminal 40 kDa subunit and a C-terminal 30 kDa subunit. It plays a role in cell proliferation, morphogenesis, and oncogenesis by interacting with signaling pathways such as Wnt, Hedgehog, and fibroblast growth factor. Abnormal expression of GPC3 is associated with poor prognosis in cancer, making it a potential target for cancer diagnosis and immunotherapy.
流式分析
Flow cytometric analysis of Human Glypican-3 expression on HepG2 cells. Cells from the HepG2 (Human hepatocellular carcinoma epithelial cell, Right) or A549 (Human lung carcinoma epithelial cell, Left) was stained with either APC Rabbit IgG Isotype Control (Black line histogram) or SDT APC Rabbit Anti-Human Glypican-3 Antibody (Red line histogram) at 5 μl/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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