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Rat抗原名称
CD45RA分子别名
Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen (L-CA); Lymphocyte antigen 5 (Ly-5); T200; CD45; Ly-5; Ptprc细胞定位
Cell membrane, SynapseAccession
P06800克隆号
14.8抗体类型
Rat mAb抗体同种型
IgG2b,k反应种属 ?
Ms阳性样本
BALB/c mouse splenocytes纯化方式
Protein G浓度
0.05mg/ml标记
FITC性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Ms
D45RA is an isoform of the CD45 protein, a member of the protein tyrosine phosphatase (PTP) family, and serves as a critical regulator of T- and B-cell antigen receptor signaling. It is predominantly expressed on the surface of B cells, from early pro-B cells to mature and activated B cells, making it a commonly used pan-B cell marker in mice. Additionally, CD45RA is also found on certain subsets of activated T cells, lymphokine-activated killer cells (LAK), NK cell progenitors, and T cells in mutant mice. This molecule plays a crucial role in lymphocyte development and antigen signaling, and its expression is regulated during different stages of B cell maturation.
流式分析
Flow cytometric analysis of Mouse CD45RA expression on BALB/c mouse splenocytes. BALB/c mouse splenocytes were stained with Brilliant Violet 421™ Rat Anti-Mouse CD3 Antibody and either FITC Rat IgG2b, κ Isotype Control (Left panel) or SDT FITC Rat Anti-Mouse CD45RA Antibody (Right panel) at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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