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Mouse抗原名称
CD20分子别名
B-lymphocyte antigen CD20; B-lymphocyte surface antigen B1; Bp35; Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 1; MS4A1细胞定位
Cell membraneAccession
P11836克隆号
2H7抗体类型
Mouse mAb抗体同种型
IgG2b,k反应种属 ?
RhMk, CyMk, Hu阳性样本
human peripheral blood cells纯化方式
Protein A浓度
0.2 mg/ml标记
APC-Cy7性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Hu
CD20, also known as B-lymphocyte antigen CD20, is a 33–37 kDa non-glycosylated protein primarily expressed on the surface of B cells from the pre-B cell stage through maturity, but it is absent on plasma cells. It is encoded by the MS4A1 gene and belongs to the membrane-spanning 4A gene family. CD20 is a transmembrane protein with four hydrophobic transmembrane domains, one intracellular domain, and two extracellular loops. Although its exact biological function remains unclear, it is believed to play a role in B cell activation and proliferation by facilitating calcium influx. CD20 is an important therapeutic target for B cell malignancies such as chronic lymphocytic leukemia and non-Hodgkin lymphoma, as well as for some autoimmune diseases.
流式分析
Flow cytometric analysis of Human CD20 expression on human peripheral blood cells. Human peripheral blood cells were stained with PE-Cy7 Mouse Anti-Human CD19 Antibody and either APC-Cy7 Mouse IgG2b, κ Isotype Control (Left panel) or SDT APC-Cy7 Mouse Anti-Human CD20 Antibody (Right panel) at 5 μl/test and treated with True-Stain Monocyte Blocker™ separately. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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