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Mouse抗原名称
CD20分子别名
B-lymphocyte antigen CD20; B-lymphocyte surface antigen B1; Bp35; Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 1; MS4A1细胞定位
Cell membraneAccession
P11836克隆号
2H7抗体类型
Mouse mAb抗体同种型
IgG2b,k反应种属 ?
RhMk, CyMk, Hu阳性样本
human peripheral blood cells纯化方式
Protein A浓度
0.05mg/ml标记
Alexa Fluor® 488性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Hu
CD20, also known as B-lymphocyte antigen CD20, is a 33–37 kDa non-glycosylated protein primarily expressed on the surface of B cells from the pre-B cell stage through maturity, but it is absent on plasma cells. It is encoded by the MS4A1 gene and belongs to the membrane-spanning 4A gene family. CD20 is a transmembrane protein with four hydrophobic transmembrane domains, one intracellular domain, and two extracellular loops. Although its exact biological function remains unclear, it is believed to play a role in B cell activation and proliferation by facilitating calcium influx. CD20 is an important therapeutic target for B cell malignancies such as chronic lymphocytic leukemia and non-Hodgkin lymphoma, as well as for some autoimmune diseases.
流式分析
Flow cytometric analysis of Human CD20 expression on human peripheral blood cells. Human peripheral blood cells were stained with Brilliant Violet 421™ Mouse Anti-Human CD19 and either Alexa Fluor® 488 Mouse IgG2b, κ Isotype Control (Left panel) or SDT Alexa Fluor® 488 Mouse Anti-Human CD20 Antibody (Right panel) at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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