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宿主来源
Armenian hamster抗原名称
CD27分子别名
Signal transducer CD24; Lymphocyte antigen 52 (Ly-52); M1/69-J11D heat stable antigen (HSA); Nectadrin; R13-Ag; X62 heat stable antigen; Cd24a; Ly-52细胞定位
Cell membraneAccession
P26842, P41272, Q501W2克隆号
S-R620抗体类型
Recombinant mAb反应种属 ?
Hu, Ms, Rt纯化方式
Protein G浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1:200 Hu, Ms, Rt
CD27 is a transmembrane protein that belongs to the tumor necrosis factor receptor superfamily (TNFRSF) and is primarily expressed on T cells, B cells, and natural killer (NK) cells. It activates NF-κB and JNK signaling pathways by binding to its ligand, CD70, thereby promoting the activation, proliferation, and differentiation of T cells. CD27 plays a crucial role in immune regulation, acting as an important co-stimulatory receptor for T and B cells, and is involved in modulating immune responses and the formation of memory cells. Additionally, CD27 is being studied as a potential target for anti-tumor immunotherapy.
流式分析
Flow cytometric analysis of human peripheral blood cells labelling CD27 antibody at 1/200 (1 μg) dilution/ (Right panel) compared with a Amenian hamster IgG Isotype Control / (left panel). Goat Anti-Amenian hamster IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD19 - Brilliant Violet 421™ antibody at separately.
Flow cytometric analysis of C57BL/6 mouse splenocytes labelling CD27 antibody at 1/200 (1 μg) dilution/ (Right panel) compared with a Amenian hamster IgG Isotype Control / (left panel). Goat Anti-Amenian hamster IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD19 - Brilliant Violet 421™ antibody at separately.
Flow cytometric analysis of SD Rat splenocytes labelling CD27 antibody at 1/200 dilution (1 μg) / (Red) compared with a Amenian hamster IgG (Black) Isotype Control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Amenian hamster IgG Alexa Fluor® 488 was used as the secondary antibody.
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