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Rat抗原名称
CD45RA分子别名
Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen (L-CA); Lymphocyte antigen 5 (Ly-5); T200; CD45; Ly-5; Ptprc细胞定位
Cell membrane, SynapseAccession
P06800克隆号
14.8抗体类型
Rat mAb抗体同种型
IgG2b,k反应种属 ?
Ms阳性样本
BALB/c mouse splenocytes纯化方式
Protein G浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1:2000 Ms
CD45RA is an isoform of the CD45 protein, a member of the protein tyrosine phosphatase (PTP) family, and serves as a critical regulator of T- and B-cell antigen receptor signaling. It is predominantly expressed on the surface of B cells, from early pro-B cells to mature and activated B cells, making it a commonly used pan-B cell marker in mice. Additionally, CD45RA is also found on certain subsets of activated T cells, lymphokine-activated killer cells (LAK), NK cell progenitors, and T cells in mutant mice. This molecule plays a crucial role in lymphocyte development and antigen signaling, and its expression is regulated during different stages of B cell maturation.
流式分析
Flow cytometric analysis of BALB/c mouse splenocytes labelling Mouse CD45RA antibody at 1/2000 dilution (0.1 μg) / (Right panel) compared with a Rat IgG2b, κ Isotype Control / (Left panel). Goat Anti-Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD3 - Phycoerythrin Antibody separately.
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