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宿主来源
Mouse抗原名称
Siglec-2/CD22分子别名
B-cell receptor CD22; B-lymphocyte cell adhesion molecule (BL-CAM); Sialic acid-binding Ig-like lectin 2 (Siglec-2); T-cell surface antigen Leu-14; SIGLEC2免疫原
Recombinant Protein细胞定位
Cell membraneAccession
P20273克隆号
S-565-1抗体类型
Mouse mAb抗体同种型
IgG1,k反应种属 ?
Hu阳性样本
human peripheral blood lymphocytes纯化方式
Protein G浓度
0.05mg/ml标记
FITC性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5 μl per million cells in 100μl volume Hu
Siglec-2, also known as CD22, is a type I transmembrane protein belonging to the immunoglobulin superfamily and functions as an inhibitory lectin on B cells. It is characterized by its ability to bind α2,6-linked sialic acid on the same (cis) or adjacent (trans) cells. CD22 plays a crucial role in mediating B cell interactions, directing B cell localization within lymphoid tissues, and regulating B cell antigen receptor signaling. The protein contains four immunoreceptor tyrosine-based inhibitory motifs (ITIMs), allowing it to recruit cytoplasmic phosphatases to inhibit downstream signaling pathways. Additionally, CD22 can form cis interactions with sialylated glycoproteins such as CD45, potentially masking its sialic acid recognition site. Its complex interactions with various signaling molecules highlight its multifaceted role in B cell regulation and immune responses.
流式分析
Flow cytometric analysis of Human CD22 expression on human peripheral blood lymphocytes. Human peripheral blood lymphocytes were stained with Brilliant Violet 421™ Mouse Anti-Human CD19 Antibody and either Mouse IgG1, κ Isotype Control (Left panel) or SDT FITC Mouse Anti-Human Siglec-2/CD22 Antibody (Right panel) at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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