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宿主来源
Rabbit抗原名称
CD14分子别名
Monocyte differentiation antigen CD14; My23 antigen; Myeloid cell-specific leucine-rich glycoprotein免疫原
Synthetic Peptide细胞定位
Cell membrane, Secreted, Golgi ApparatusAccession
P08571克隆号
S-395-8抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
human PBMC纯化方式
Protein A浓度
0.2 mg/ml标记
Pacific Blue性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1.25μl per million cells in 100μl volume Hu
CD14 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein primarily expressed on monocytes, macrophages, and some granulocytes. It functions as a pattern recognition receptor (PRR) and plays a crucial role in the innate immune response by recognizing bacterial lipopolysaccharides (LPS), pathogen-associated molecular patterns (PAMPs), and damage-associated molecular patterns (DAMPs). CD14 exists in two forms: membrane-bound CD14 (mCD14) and soluble CD14 (sCD14), which can be secreted by activated cells. It acts as a co-receptor for Toll-like receptor 4 (TLR4) and enhances the immune response to LPS by facilitating its delivery to the TLR4-MD2 complex. Additionally, CD14 is involved in TLR4-independent signaling pathways and contributes to tissue homeostasis by promoting the clearance of apoptotic cells.
流式分析
Flow cytometric analysis of Human CD14 expression on human PBMC (human peripheral blood mononuclear cells). Human PBMC were stained with Pacific Blue™ Rabbit IgG Isotype Control (Left panel) or SDT Pacific Blue Rabbit Anti-Human CD14 Antibody (Right panel) at 1.25μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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