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宿主来源
Mouse抗原名称
CD25分子别名
Peripheral myelin protein 22; PMP-22; Protein CD25; SR13 myelin protein; Schwann cell membrane glycoprotein (SAG); Pmp22细胞定位
Cell membraneAccession
P25094克隆号
OX-39抗体类型
Mouse mAb抗体同种型
IgG1,k反应种属 ?
Rt阳性样本
BALB/c mouse splenocytes treated overnight with 3μg/ml Concanavalin A纯化方式
Protein G浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1:2000 Rt
CD25, also known as the interleukin-2 receptor alpha chain (IL-2Rα), is a membranous glycoprotein that forms the alpha chain of the IL-2 receptor complex. It is primarily expressed on activated T lymphocytes and plays a crucial role in regulating immune responses by mediating IL-2 signaling. When combined with IL-2Rβ and the common γ chain, CD25 forms a high-affinity receptor complex that enhances the binding and signaling of IL-2, promoting T-cell proliferation and activation. Additionally, CD25 is involved in the development and function of regulatory T cells, which help maintain immune tolerance and prevent autoimmune diseases. Abnormal expression of CD25 has been associated with various diseases, including autoimmune disorders and certain cancers, making it a potential target for therapeutic interventions.
流式分析
Flow cytometric analysis of BALB/c mouse splenocytes treated overnight with 3μg/ml Concanavalin A (Right) or untreated (Left), labeling CD25 at 1/2000 dilution (0.1 μg). Goat Anti - Rat IgG Alexa Fluor® 647 was used as the secondary antibody. Then cells were stained with CD4 - Alexa Fluor® 488 separately. Gated on total viable cells.
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