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宿主来源
Rat抗原名称
Ly6G分子别名
Lymphocyte antigen 6G; Ly-6G; Ly-6G.1; Ly6g细胞定位
Cell membraneAccession
P35461克隆号
RB6-8C5抗体类型
Rat mAb抗体同种型
IgG2b,k反应种属 ?
Ms阳性样本
C57BL/6 mouse bone marrow纯化方式
Protein G浓度
0.2 mg/ml标记
APC性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1.25μl per million cells in 100μl volume Ms
Ly6G (Lymphocyte antigen 6 complex locus G6D) is a 21-25 kDa glycosylphosphatidylinositol (GPI)-anchored protein that belongs to the Ly-6 superfamily and is expressed in a developmentally regulated manner by myeloid cells, including neutrophils, granulocytes, and some monocytes. It serves as a key marker for mature neutrophils and is widely used in research to identify and deplete these cells in vivo. Ly6G is transiently expressed on monocytes during bone marrow development, while its expression on granulocytes and peripheral neutrophils correlates with their differentiation and maturation levels. This antigen plays important roles in immune responses, such as antitumor immunity and neutrophil-mediated inflammation.
流式分析
Flow cytometric analysis of Mouse Ly-6G/Ly-6C (Gr-1) expression on C57BL/6 mouse bone marrow. C57BL/6 mouse bone marrow was stained with Brilliant Violet 510™ Rat Anti-Mouse CD11b Antibody and either APC Rat IgG2b, κ Isotype Control (Left panel) or SDT APC Rat Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody (Right panel) at 1.25μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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